The staining mix has to be prepared fresh each time and to be used for staining cells immediately after addition of ascorbate. Description EdU (5-ethynyl-2’-deoxyuridine) can be used as a replacement for BrdU (5-bromo-2’-deoxyuridine) and directly measures de novo DNA synthesis or S-phase synthesis of the cell cycle using click chemistry.
After staining, wash the cells on coverslips several times with TBS with 0.5% Triton X-100. We use cookies to make our site as useful as possible. If you continue without changing your cookie settings, we'll assume you’re happy with this. 10EdU staining of proliferating cells.
Dual pulse labeling with EdU and BrdU. Golgi (in green) is labeled with Alexa Fluor® 488 conjugate of lectin HPA, and peroxisomes (in orange) are labeled with a rabbit primary followed by Alexa Fluor® 555 donkey anti–rabbit IgG antibody.
Labeled DNA detected using Click-iT® EdU Alexa Fluor® 647 kit (Cat no. HeLa (Human epithelial cell line from cervix adenocarcinoma) cells (4 x 10There are currently no Customer reviews or Questions for
C10085).
A fluorescent azide, such as iFluor-488, is then added. Click chemistry is a method of covalently coupling an azide with an alkyne. | Cell proliferation detected with Click-iT® EdU and counter stained with anti-LC3B and a goat anti-rabbit Alexa Fluor® 647 secondary antibody.Using anti-LC3B and the Click-iT® technology autophagy detected and glycoproteins labeled in HeLa cellsProliferating cells labeled in vivo with the Click-iT® EdU assay.Multicolor imaging with Click-iT® EdU. In EdU staining, EdU is incorporated into newly synthesized DNA by cells within a sample. Muntjac cells pulsed with EdU, fixed and permed. If incorrect, please enter your country/region into the box below, to view site information related to your country/region.Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.Access advice and support for any research roadblockFull event breakdown with abstracts, speakers, registration and moreGuaranteed product quality, expert customer support.EdU Assay / EdU Staining Proliferation Kit (iFluor 488) ab219801 provides a sensitive and robust method to detect and quantify cell proliferation in live mammalian cells using flow cytometry or fluorescence microscopy. EdU Assay / EdU Staining Proliferation Kit (iFluor 647) ab222421 provides a sensitive and robust method to detect and quantify cell proliferation in live mammalian cells using flow cytometry or fluorescence microscopy. The Click-iT® EdU Flow Cytometry Assay Kits are novel alternatives to the BrdU assay. EdU (5-ethynyl-2'-deoxyuridine) is a nucleoside analog of thymidine and is incorporated into DNA during active DNA synthesis. The iFluor 488 dye (Ex/Em: 491/520 nm) has spectral properties almost identical to those of FITC and alternative green fluorophores.EdU staining protocol summary (wash cells between each step):EdU staining can also be combined with antibody staining or cell staining with other fluorescent dyes.This kit provides enough reagents to perform 50 flow cytometry tests or 50 microscopy tests (for 18 x 18 mm coverslips) or 200 microscopy tests (adapted for 96-well plate format).Previously called EdU Proliferation Assay Kit (iFluor 488).The most accurate method to measure DNA proliferation is by directly measuring DNA synthesis. For Research Use Only. Figure 5. Each kit contains enough material for 50 to 250 reactions, depending on reaction volume (typically between 0.1 and 0.5 ml). EdU Assay / EdU Staining Proliferation Kit (iFluor 488)RP11-284F21.9 promotes oral squamous cell carcinoma development via the miR-383-5p/MAL2 axis.Liraglutide protects renal mesangial cells against hyperglycemia-mediated mitochondrial apoptosis by activating the ERK-Yap signaling pathway and upregulating Sirt3 expression. *Please select more than one item to compare
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Tubulin (in blue) is labeled with a mouse primary followed by Alexa Fluor® 350 goat anti–mouse IgG antibody. EdU (5-ethynyl-2´-deoxyuridine) is a nucleoside analog to thymidine and is incorporated into DNA during active DNA synthesis. In this assay, the modified thymidine analog EdU (5-ethynyl-2'-deoxyuridine, a nucleoside analog of thymidine) is efficiently incorporated into newly synthesized DNA and fluorescently labeled with a bright, photostable Alexa Fluor® dye in a fast, highly-specific, mild click reaction This protocol can be used for: Search results for EdU antibody at Sigma-Aldrich. HeLa (Human epithelial cell line from cervix adenocarcinoma) cells (4 x 10Dot plot of EdU-488 staining (Y-axis, 488) vs FSC.
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